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    公开号:AU2003266075A1
    申请号:U2003266075
    申请日:2003-09-19
    公开日:2004-04-08
    发明作者:Martin Gagnon；H. Uri Saragovi
    申请人:McGill University；
    IPC主号:A61K38-00
    专利说明:
    WO 2004/026895 PCT/CA2003/001389 GD2 LIGANDS FIELD OF THE INVENTION The invention includes therapeutic compositions and methods, such as 5 peptide cancer chemotherapeutic agents. BACKGROUND OF THE INVENTION Gangliosides are cell surface glycosphingolipids containing one or more sialic acid residues. It has been suggested that gangliosides may be localized 10 within detergent-resistant cell membrane microdomains termed "rafts", which may provide the environment for some proteins to function by bringing together adapter molecules, modifiers, substrates, or cofactors that would be otherwise too distant or too dilute to form complexes and activate a signal cascade. However, little has been proposed regarding the possible mechanism of action of gangliosides in 15 signal transduction. Ganglioside GD2 is reportedly expressed at low levels in certain neuronal populations, but is highly prevalent in many types of tumors (neuroblastoma, melanoma, small cell carcinoma of the lung, gliomas, soft tissue sarcomas and B 20 cell lymphoma). The extracellular matrix component Tenascin-R was described recently as a natural ligand for GD2 (Probstmeier et al., 1999). However, relatively little is known about the biological function(s) of GD2 and the functional nature of its interaction 25 with ligands such as Tenascin-R. GD2 has been extensively studied as a tumor marker and is used clinically as a target for antibody-mediated therapy (e.g. anti-GD2 mAb 3F8) (Cheung et al., 1985). However, anti-GD2 mAb 3F8 applied therapeutically to patients causes 30 acute and transient pain immediately after administration. Anti-GD2 mAb 3F8 based therapeutics have been suggested for use in a wide range of cancer -1- WO 2004/026895 PCT/CA2003/001389 therapeutics and diagnostics, including neuroblastoma and leptomeningeal cancer. For example, 1311 I-labeled anti-GD2 3F8 monoclonal antibody has been used in targeted radioimmunotherapy (dosed at 20 mCi/kg) in conjunction with immunotherapy with 400 mg/m2 unlabeled/unmodified 3F8. Similarly, granulocyte 5 macrophage colony-stimulating factor (GM-CSF) has been used in conjunction with anti-GD2 monoclonal antibody 3F8 in the treatment of patients with neuroblastoma. SUMMARY OF THE INVENTION In one aspect, the invention provides GD2 ligands of Formula I: 10 Zi-XI-X 2
    -X
    3
    -X
    4 -Xs-X 6
    -X
    7
    -X
    8 -- XI-Xll-X1 2 -X1 3
    -Z
    2 (I) wherein
    X
    1 is absent or Y or an analogue thereof; 15 X 2 is absent or C or an analogue thereof;
    X
    3 is G or Y or an analogue thereof;
    X
    4 is G or C or Y or an analogue thereof;
    X
    5 is I or C or an analogue thereof;
    X
    6 is T or A or an analogue thereof; 20 X 7 is N or an analogue thereof;
    X
    8 is Y or an analogue thereof;
    X
    9 is N or G or an analogue thereof;
    X
    10 is S or C or V or T or an analogue thereof;
    X
    11 is A or C or Y or H or S or an analogue thereof; 25 X 12 is absent or L or C or Y or an analogue thereof;
    X
    13 is absent or M or Y or an analogue thereof;
    Z
    1 is an N-terminal group of the formula H2N-, RHN- or, RRN-;
    Z
    2 is a C-terminal group of the formula -C(O)OH, -C(O)R, -C(O)OR, C(O)NHR, -C(O)NRR; 30 R at each occurrence is independently selected from (C 1
    -C
    6 ) alkyl,
    (C
    1
    -C
    6 ) alkenyl, (C 1
    -C
    6 ) alkynyl, substituted (C 1
    -C
    6 ) alkyl, substituted (C01-C6) alkenyl, or substituted (C1-C6) alkynyl; and wherein "-" is a covalent linkage. -2- WO 2004/026895 PCT/CA2003/001389 In alternative embodiments, the invention provides substantially pure synthetic GD2 ligands or recombinant GD2 ligands having a domain of Formula II: 5 -X 1
    -X
    2
    -X
    3
    -X
    4
    -X
    5
    -X
    6
    -X
    7
    -X-X
    9
    -X
    1
    -X
    1 1
    -X
    12
    -X
    13 -(1l) wherein
    X
    1 is absent or Y or an analogue thereof;
    X
    2 is absent or C or an analogue thereof; 10 X 3 is G or Y or an analogue thereof;
    X
    4 is G or C or Y or an analogue thereof;
    X
    5 is I or C or an analogue thereof;
    X
    6 is T or A or an analogue thereof;
    X
    7 is N or an analogue thereof; 15 X 8 is Y or an analogue thereof;
    X
    9 is N or G or an analogue thereof;
    X
    10 is S or C or V or T or an analogue thereof;
    X
    11 i is A or C or Y or H or S or an analogue thereof;
    X
    12 is absent or L or C or Y or an analogue thereof; 20 X 13 is absent or M or Y or an analogue thereof; and wherein "-" is a covalent linkage. In one aspect, the invention provides recombinant proteins having domains of the invention, wherein the domain is capable of mediating binding of the 25 recombinant protein to GD2. For example, recombinant T-cell receptors having the domains of the invention may be provided in transformed T-cell lines, such as cytotoxic T-cells (a "cytotoxic T lymphocyte" or "CTL" is an immune system cell that recognises epitopes presented by class I MHC molecules through its TCR.). Transformed T-cell lines of the invention may for example be used to treat diseases 30 such as cancers having pathological tissues characterized by expression of GD2 (similar to an approach described in United States Patent Application 20020018783 -3- WO 2004/026895 PCT/CA2003/001389 A1, published in the name of Sadelain, M. et al. on February 14, 2002, incorporated herein by reference). The GD2 ligands of the invention may further comprise a cyclic linkage 5 between any two of X 1 through X 13 . In alternative embodiments, the GD2 ligands of the invention, or the domains of the invention, may be selected from the group consisting of: GGITNYNSALM; YCGGITNYNSACY; YCITNYNSCY; YCGGITNYNCY; YCTNYGVHCY; YCTNYGVCY; GGIANYNTS; YCGGIANYNCY; YCGGIANYNTSCY; and, YCIANYNTCY. In some embodiments, known GD2 10 ligands such as mAb 3F8 and tenascin-R are specifically excluded from the genus of claimed ligands in the present invention. However, in some embodiments, small molecule derivatives and analogs of known ligands (such as small peptides or peptidomimetics of the complementarity determining region of mAbs) are not excluded. 15 The invention also provides methods of treating a subject having a disease wherein disease cells express GD2, the method comprising administering to the subject an effective amount of the GD2 ligands of the invention. Also provided are methods of diagnosis of a disease wherein disease cells express GD2, comprising 20 determining whether a cell from a subject binds to a GD2 ligand of the invention. The diagnostic and therapeutic methods of the invention may be carried out in vitro or in vivo. In alternative embodiments, the GD2 ligands of the invention may be used 25 with other therapeutic compounds, such as an effective amount of granulocyte macrophage colony-stimulating factor. The invention provides commercial packages comprising the GD2 ligands of the invention, together with instructions for using the GD2 ligands to modulate GD2 activity or to detect cells expressing GD2. 30 Table 1 sets out the sequences of a number of alternative GD2 ligands or GD2 binding domains of the invention. Structure activity relationship (SAR) and -4- WO 2004/026895 PCT/CA2003/001389 deletion analysis demonstrate that some substitutions are allowed and that peptide cyclization enhances activity (peptides marked by * are inactive) Table 1: Alternative domainslpeptides of the invention G G I T N Y N S A L M Y C G G I T N Y N S A C Y Y C I T N Y N S C Y Y C G G I T N Y N C Y Y C T N Y G V H C Y * Y C T N Y G V C Y * G G I A N Y N T S * Y C G G I A N Y N C Y Y C G G I A N Y N T S C Y Y C I A N Y N T C Y Xl X 2
    X
    3
    X
    4
    X
    5
    X
    6
    X
    7
    X
    8
    X
    9
    XI
    0
    X
    11
    X
    12
    X
    13 5 The invention further provides commercial packages comprising a GD2 ligand, together with instructions for using the GD2 ligand, for example by administering the GD2 ligand to a patient in need of such treatment. 10 In alternative embodiments, the invention provides methods of screening to identify a GD2 ligand. For example, such screening methods may involve: a) Administering a putative GD2 ligand (such as a polypeptide derived from Tenascin-R) to a system having a GD2 moiety and a p 5 6 Lck moiety available for association (such as a cell expressing GD2 and p 56 Lck); and, 15 b) Measuring an association between the GD2 and the p5 6 Lck moieties in the system, for example by determining a kinase activity of the p 5 6 Lck moiety. In further alternative embodiments, the invention may include: c) Measuring an association between the GD2 and the CD45 moieties in the system, for example by determining the phosphatase activity of the 20 CD45 moiety. -5- WO 2004/026895 PCT/CA2003/001389 In further alternative embodiments, the forgoing method further comprises: b) Administering a putative GD2 ligand to a system having a GD2 moiety and a phosphatase such as CD45; and c) Measuring an association or functional relationship between 5 GD2 and the phosphatase d) A competitive screening assay where test compounds (e.g. a combinatorial library) are used to displace any of the GD2 ligands described herein e) A competitive screening assay where any of the GD2 ligands 10 described herein are used to displace test compounds In some embodiments, the putative GD2 ligand may for example comprise a polypeptide or a non-peptidic analog such as a peptidomimetic that displays the same pharmacophore or has similar side chain functional groups. 15 BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is the full-length protein sequence of Homo sapiens tenascin-R 20 (restrictin, janusin), from GenBank Accession XP_040550, with a region identified in bold within a box that is identified in accordance with one aspect of the invention as a GD2 binding region. One aspect of the invention provides alternative tenascin R proteins in which the GD2 binding region has been altered, for example to modulate the strength of the interaction between GD2 and the tenascin-R protein. 25 Figure 2 shows data relating to the co-immunoprecipitation of GD2 and p 5 6 LCk: (a) R1.1 and EL4 GD2positive cell lysates were subjected to immunoprecipitation with anti-GD2 mAb 3F8 or control mouse IgG (mlg). Presence 30 of p 5 6 Lck protein in the immunoprecipitates was evaluated by western blotting. p 5 6 Lck can be immunoprecipitated by mAb 3F8 in EL4 GD2PO s itive cells (lane 7) but not in R1.1 cells (lane 5). Total cell lysates were used as a control for p 5 6 Lck detection (lane 1 and 2). -6- WO 2004/026895 PCT/CA2003/001389 (b) and (c) R1.1, EL4 GD2nega ti ve and EL4 GD2po s i tive cell lysates were subjected to immunoprecipitation with anti-GD2 mAb 3F8, GM1 -binding cholera toxin B, anti-p56Lck mAb 3A5 and anti-Zap-70 mAb LR. Presence of p 5 6 Lck kinase activity in the immunoprecipitates was evaluated by in vitro kinase assay with the 5 p56Lck-specific substrates Sam68 and Gap p62. The data in Figure 2 (b) shows that p 5 6 Lck kinase activity can be immunoprecipitated by mAb 3F8 in EL4 GD2positive cells (lane 1) but not in EL4 GD2negative cells (lane 4). The data in Figure 2 (c) shows that p 5 6 Lck kinase activity can be immunoprecipitated by mAb 3F8 in EL4 GD2posi ti ve cells (lane 1) but by cholera toxin B in R1.1 cells (lane 4). 10 (d) R1.1 and EL4 GD2po si tive cell lysates were subjected to immunoprecipitation with anti-GD2 mAb 3F8, GMI -binding cholera toxin B and anti p 5 6 Lck mAb 3A5. Presence of gangliosides GD2 and GM1 in the immunoprecipitates was evaluated by ELISA. Anti-p56Lck mAb 3A5 can immunoprecipitate GD2 from EL4 GD 2 po s i tive cells, but can not immunoprecipitate 15 GM1 from R1.1 cells. Shown are averages ± SEM of one representative experiment. n=4 per experiment. Figure 3 shows the effect of gangliosides on the in vitro kinase activity of purified p 5 6 Lck . The kinase activity of p 5 6 Lck was evaluated by measuring the 20 incorporation of 32 P0 4 in a peptidic p 5 6 Lck substrate over time, with or without GD2 or GM1. Presence of GD2, but not GM1, can positively alter the kinetics of p 5 6 Lck kinase activity. Activity standardized to untreated p 5 6 Lck at the 20 minute time point. Shown are averages of 3 to 5 assays ± SEM. 25 Figure 4 shows: (a) the effect of GD2 ligands on p 5 6 Lck phosphorylation. Resting EL4 GD2O siti ve cells were treated with mAb 3F8 (13 nM) or control mouse IgG (mlg) for the indicated times. After lysis, p56 L ck protein was immunoprecipitated with anti p56 L c k mAb 3A5 and probed for phosphotyrosine (PY) by western blotting using 30 biotinylated anti-phosphotyrosine mAb 4G10. Anti-GD2 mAb 3F8 can induce tyrosine phosphorylation of p 5 6 Lck within 5 minutes (lane 3) and sustain it for at least 30 minutes (lane 4). -7- WO 2004/026895 PCT/CA2003/001389 (b) the effect of GD2 ligands on Zap-70 phosphorylation. Resting EL4 GD2po si t ive cells were treated with mAb 3F8 (13 nM) or control mouse IgG (mlg) for the indicated times. After lysis, Zap-70 protein was immunoprecipitated with anti Zap-70 mAb LR and probed for phosphotyrosine (PY) by western blotting using 5 anti-phosphotyrosine mAb 4G10. Anti-GD2 mAb 3F8 can induce tyrosine phosphorylation of Zap-70 within 5 minutes (lane 3). c) Effect of GD2 ligands on intracellular calcium concentrations. Resting EL4 GD2po s i t i ve cells were treated with mAb 3F8 (13 nM), control mouse IgG (mig) or the calcium ionophore A23187. Intracellular calcium concentration was evaluated over time by flow cytometry using 10 the calcium-sensitive fluorophore Rhod-2 AM. mAb 3F8 can induce strong, sustainable calcium changes within 5 minutes, while control mouse IgG has no effect. Addition of the p 5 6 Lck inhibitor PP1 partially abolished mAb 3F8's effects. Shown are averages of 4 independent assays + SEM. 15 Figure 5 shows the inhibitory effect of GD2 on in vitro CD45 phosphatase activity, particularly when using the pp 6 0 src C-terminal phosphoregulatory sequence as a substrate. When co-incubated for 20 minutes, GD2 (but not other gangiosides) can drastically inhibit CD45 phosphatase activity, as measured by the colorimetric quantification of released free phosphate. 20 Figure 6 shows the inhibitory effect of GD2 on ex vivo CD45 phosphatase activity, measured by the dephosphorylation of tyrosine 505 on p 5 6 Lck. EL4
    GD
    2 posit i ve and EL4 GD 2 ne g ative cells were treated with control mlg, anti-GD2 antibody 3F8 or anti-CD3 antibody 145-2C1 1 cross-linked with anti-hamster 25 antibody G94-56 for 20 minutes at 37'C. After lysis, p 5 6 Lck was immunoprecipitated with anti-p56Lck 3A5 coated beads and probed for phosphotyrosine at position 505 by western blotting with anti-PY505 antibody. EL4 GD2po s iti ve cells treated with anti CD3 fail to induce dephosphorylation of p 5 6 Lck at tyrosine 505 (lane 3), while EL4
    GD
    2 ne g at i ve cells show a marked dephosphorylation with the same treatment (lane 30 6), indicating that the presence of GD2 inhibits the CD45 phosphatase responsible for tyrosine 505 dephosphorylation. GD2 ligands such as anti-GD2 antibody 3F8, are able to relieve CD45 of its GD2-mediated inhibition (lane 2). -8- WO 2004/026895 PCT/CA2003/001389 Figure 7 shows data from soft-agar clonogenic assays. EL4 GD 2 Po s iti ve and EL4 GD 2 negative cells were cultured in soft agar in the presence or absence of anti GD2 antibody 3F8 (2 plates per sample). 5 (a) Total number of visible colonies. EL4 GD 2 po sit ive cells show reduced colony formation compared to EL4 GD 2 ne g ative cells. Anti-GD2 antibody 3F8 abolishes EL4 GD2po si ti ve cells growth but does not affect EL4 GD2negat iv e cells. (b) Typical EL4 GD2positive and EL4 GD2negative colonies. EL4 GD2ne g at i ve colonies contain many more cells than EL4 GD2positive colonies. Bar = 100 10 micrometers. Figure 8 shows data from tumorigenic assays. EL4 GD 2 pos ti ve and EL4
    GD
    2 negat i ve cells were injected intraperitoneally in a) nude Balb/c and b) syngeneic C57BL/g mice. EL4 GD2negative injected animals (left) show important ascitic tumors, 15 whereas EL4 GD2posit i ve injected animals (right) show localized solid tumors (circled area). Bar = 1 mm. DETAILED DESCRIPTION OF THE INVENTION 20 In one aspect, the invention provides selective artificial ligands of GD2. In some embodiments, such ligands may be used to modulate ganglioside GD2 signal transduction selectively in the tissue where it is normally expressed, particularly in pathways that can be linked to tumorigenic growth or to nociceptive receptors. One aspect of the invention involves the identification of primary and tertiary structures 25 in Tenascin-R that are critical for GD2 binding. In an alternative aspect, the invention demonstrates that a complementarity determining region of anti-GD2 mAb 3F8 is an analogue of Tenascin-R. Small peptide mimics of Tenascin-R and mimics of anti-GD2 mAb 3F8 have accordingly been designed and synthesized as selective ligands of GD2. Alternative ligands may be provided, based on the ligands 30 of the invention, by protein mimicry and antibody mimicry techniques (Saragovi et al., 1992). -9- WO 2004/026895 PCT/CA2003/001389 In an alternative aspect of the invention, it is demonstrated that GD2 (but not GM1) associates physically with p 5 6 Lck in vivo, in complexes that are stable to detergent lysis. It is shown that GD2 regulates signal transduction by enhancing p56 L ck enzymatic activity in vitro and by enhancing in vivo phosphorylation of p 5 6 Lck 5 and the p 5 6 Lck substrate Zap70. Accordingly, GD2 ligands of the invention may be used to cause p56Lck-dependent fluxes in intracellular calcium in live cells, and thereby to modulate a variety of physiological or pathological cellular functions. In an alternative aspect of the invention, it is demonstrated that GD2 (but not 10 other gangliosides) can inhibit CD45 phosphatase activity in vitro and ex vivo. Accordingly, GD2 ligands of the invention may be used to antagonize the inhibitory action of GD2 on CD45 in live cells, and thereby to modulate a variety of physiological, immunological or pathological cellular functions. 15 In one aspect of the invention, expression of GD2 is shown to alter tumorigenicity in vitro and in vivo. As shown in the Examples, in EL4 clones that are otherwise phenotypically indistinguishable GD2negative than in GD2po s i ti ve cells have equal doubling times, but soft agar colony growth is significantly more efficient in GD2nega ti ve cells. Further, addition of GD2 ligands in this assay causes the apoptotic 20 death of GD 2 po s i ti ve cells. GD 2 n e g ative cells are also more tumorigenic in vivo.
    GD
    2 po si tive cells injected intraperitoneally or subcutaneously in syngeneic mice only form solid tumors at the primary site, with little metastasis. In contrast, GD 2 negative cells can digest the peritoneal membrane, form viscous, muscin-like ascitic tumors, small solid tumor nodules, and are highly metastatic. Accordingly, in one aspect of 25 the invention cells may be transformed so that they are GD 2 OSitive, and the transformed GD2positive cells may be treated to GD2 ligands of the invention to mediate cell death, for example by an apoptotic pathway. For example, GD 2 ne g at i ve cancer cells may be transformed by targeted gene therapy techniques, so that the cells become GD 2 po siti ve, and the transformed GD2po siti ve cancer cells may be 30 treated with a GD2 ligand of the invention. -10- WO 2004/026895 PCT/CA2003/001389 In alternative aspects, GD2 ligands of the invention may be used to influence or modulate signal transduction in a biologically relevant manner in vitro and in vivo in the treatment of diseases such as cancer and in managing symptoms such as pain. 5 In one aspect, the invention provides compounds, such as GD2 ligands, that are purified, isolated or substantially pure. A compound is "substantially pure" when it is separated from the components that naturally accompany it. Typically, a compound is substantially pure when it is at least 60%, more generally 75% or over 10 90%, by weight, of the total material in a sample. Thus, for example, a polypeptide that is chemically synthesised or produced by recombinant technology will generally be substantially free from its naturally associated components. A nucleic acid molecule is substantially pure when it is not immediately contiguous with (i.e., covalently linked to) the coding sequences with which it is normally contiguous in 15 the naturally occurring genome of the organism from which the DNA of the invention is derived. A substantially pure compound can be obtained, for example, by extraction from a natural source; by expression of a recombinant nucleic acid molecule encoding a polypeptide compound; or by chemical synthesis. Purity can be measured using any appropriate method such as column chromatography, gel 20 electrophoresis, HPLC, etc. The term "alkyl" refers to the radical of saturated aliphatic groups, including straight chain alkyl groups, branched-chain alkyl groups, cycloalkyl (alicyclic) groups, alkyl substituted cycloalkyl groups, and cycloalkyl substituted alkyl groups. 25 Typical alkyl groups include, but are not limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, pentyl, isopentyl, hexyl, etc. The alkyl groups can be (C1-Cs) alkyl, or (C1-C3) alkyl. A "substituted alkyl" has substituents replacing a hydrogen on one or more carbons of the hydrocarbon backbone. Such substituents can include, for example, halogen, hydroxyl, carbonyl (such as carboxyl, ketones 30 (including alkylcarbonyl and arylcarbonyl groups), and esters (including alkyloxycarbonyl and aryloxycarbonyl groups)), thiocarbonyl, acyloxy, alkoxyl, phosphoryl, phosphonate, phosphinate, amino, acylamino, amido, amidine, imino, -11 - WO 2004/026895 PCT/CA2003/001389 cyano, nitro, azido, sulfhydryl, alkylthio, sulfate, sulfonate, sulfamoyl, sulfonamido, heterocyclyl, aralkyl, or an aromatic or heteroaromatic moiety. The moieties substituted on the hydrocarbon chain can themselves be substituted, if appropriate. For instance, the substituents of a substituted alkyl may include substituted and 5 unsubstituted forms of aminos, azidos, iminos, amidos, phosphoryls (including phosphonates and phosphinates), sulfonyls (including sulfates, sulfonamidos, sulfamoyls and sulfonates), and silyl groups, as well as ethers, alkylthios, carbonyls (including ketones, aldehydes, carboxylates, and esters), -CF 3 , -ON and the like. Exemplary substituted alkyls are described below. Cycloalkyls can be further 10 substituted with alkyls, alkenyls, alkoxys, alkylthios, aminoalkyls, carbonyl substituted alkyls, -CF3, -CN, and the like. The terms "alkenyl" and "alkynyl" refer to unsaturated aliphatic groups analogous in length and possible substitution to the alkyls described above, but that 15 contain at least one double or triple bond respectively. An "alkenyl" is an unsaturated branched, straight chain, or cyclic hydrocarbon radical with at least one carbon-carbon double bond. The radical can be in either the cis or trans conformation about the double bond(s). Typical alkenyl groups include, but are not limited to, ethenyl, propenyl, isopropenyl, butenyl, isobutenyl, tert-butenyl, pentenyl, 20 hexenyl, etc. An "alkynyl" is an unsaturated branched, straight chain, or cyclic hydrocarbon radical with at least one carbon-carbon triple bond. Typical alkynyl groups include, but are not limited to, ethynyl, propynyl, butynyl, isobutynyl, pentynyl, hexynyl, etc. 25 A "substantially identical" sequence is an amino acid or nucleotide sequence that differs from a reference sequence only by one or more conservative substitutions, as discussed herein, or by one or more non-conservative substitutions, deletion, or insertions located at positions of the sequence that do not destroy the biological function of the test compound. Such a sequence can be at 30 least 60% or 75%, or more generally at least 80%, 85%, 90%, or 95%, or as much as 99% identical at the amino acid or nucleotide level to the sequence used for comparison. Sequence identity can be readily measured using publicly available sequence analysis software (e.g., Sequence Analysis Software Package of the -12- WO 2004/026895 PCT/CA2003/001389 Genetics Computer Group, University of Wisconsin Biotechnology Center, 1710 University Avenue, Madison, Wis. 53705, or BLAST software available from the National Library of Medicine). Examples of useful software include the programs, Pile-up and PrettyBox. Such software matches similar sequences by assigning 5 degrees of homology to various substitutions, deletions, substitutions, and other modifications. In alternative embodiments, GD2 ligands can be produced by substitution of either/or (i) side chains, (ii) backbone, or (iii) ionic interaction within a peptide. 10 Additionally, structural or functional analogs can include 1) homologs of the peptidic GD2 ligands generated by peptidomimicry and 2) analogs where the sequence/structure of the GD2 ligands is introduced in a larger protein to convey GD2 binding to that protein. 15 In one aspect the invention provides nucleic acids that encode peptide compounds of the invention. Such nucleic acids may be introduced into cells for expression using standard recombinant techniques for stable or transient expression. Nucleic acid molecules of the invention may include any chain of two or more nucleotides including naturally occurring or non-naturally occurring 20 nucleotides or nucleotide analogues. Various genes and nucleic acid sequences of the invention may be recombinant sequences. The term "recombinant" means that something has been recombined, so that when made in reference to a nucleic acid construct the term 25 refers to a molecule that is comprised of nucleic acid sequences that are joined together or produced by means of molecular biological techniques. The term "recombinant" when made in reference to a protein or a polypeptide refers to a protein or polypeptide molecule which is expressed using a recombinant nucleic acid construct created by means of molecular biological techniques. The term 30 "recombinant" when made in reference to genetic composition refers to a gamete or progeny or cell or genome with new combinations of alleles that did not occur in the parental genomes. Recombinant nucleic acid constructs may include a nucleotide sequence which is ligated to, or is manipulated to become ligated to, a nucleic acid -13- WO 2004/026895 PCT/CA2003/001389 sequence to which it is not ligated in nature, or to which it is ligated at a different location in nature. Referring to a nucleic acid construct as 'recombinant' therefore indicates that the nucleic acid molecule has been manipulated using genetic engineering, i.e. by human intervention. Recombinant nucleic acid constructs may 5 for example be introduced into a host cell by transformation. Such recombinant nucleic acid constructs may include sequences derived from the same host cell species or from different host cell species, which have been isolated and reintroduced into cells of the host species. Recombinant nucleic acid construct sequences may become integrated into a host cell genome, either as a result of the 10 original transformation of the host cells, or as the result of subsequent recombination and/or repair events. In one aspect the invention provides antibodies that recognize compounds of the invention, and anti-idiotypic antibodies that in turn recognize such antibodies. 15 The compounds of the invention can be used to prepare antibodies to GD2 ligands using standard techniques of preparation as, for example, described in Harlow and Lane (Antibodies; A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1988), or known to those skilled in the art. Antibodies can be tailored to minimise adverse host immune response by, for example, using chimeric 20 antibodies contain an antigen binding domain from one species and the Fc portion from another species, or by using antibodies made from hybridomas of the appropriate species. Compounds of the invention can be prepared, for example, by replacing, 25 deleting, or inserting an amino acid residue of a GD2 ligand or domain of the invention, with other conservatived amino acid residues, i.e., residues having similar physical, biological, or chemical properties, and screening for biological function. It is well known in the art that some modifications and changes can be made in the structure of a polypeptide without substantially altering the biological 30 function of that peptide, to obtain a biologically equivalent polypeptide. The peptides, ligands and domains of the present invention also extend to biologically equivalent peptides, ligands and domains that differ from a portion of the sequence -14- WO 2004/026895 PCT/CA2003/001389 of novel ligands of the present invention by conservative amino acid substitutions. As used herein, the term "conserved amino acid substitutions" refers to the substitution of one amino acid for another at a given location in the peptide, where the substitution can be made without substantial loss of the relevant function. In 5 making such changes, substitutions of like amino acid residues can be made on the basis of relative similarity of side-chain substituents, for example, their size, charge, hydrophobicity, hydrophilicity, and the like, and such substitutions may be assayed for their effect on the function of the peptide by routine testing. 10 In some embodiments, conserved amino acid substitutions may be made where an amino acid residue is substituted for another having a similar hydrophilicity value (e.g., within a value of plus or minus 2.0), where the following may be an amino acid having a hydropathic index of about -1.6 such as Tyr (-1.3) or Pro (-1.6)s are assigned to amino acid residues (as detailed in United States 15 Patent No. 4,554,101, incorporated herein by reference): Arg (+3.0); Lys (+3.0); Asp (+3.0); Glu (+3.0); Ser (+0.3); Asn (+0.2); GIn (+0.2); Gly (0); Pro (-0.5); Thr ( 0.4); Ala (-0.5); His (-0.5); Cys (-1.0); Met (-1.3); Val (-1.5); Leu (-1.8); le (-1.8); Tyr (-2.3); Phe (-2.5); and Trp (-3.4). 20 In alternative embodiments, conserved amino acid substitutions may be made where an amino acid residue is substituted for another having a similar hydropathic index (e.g., within a value of plus or minus 2.0). In such embodiments, each amino acid residue may be assigned a hydropathic index on the basis of its hydrophobicity and charge characteristics, as follows: lie (+4.5); Val (+4.2); Leu 25 (+3.8); Phe (+2.8); Cys (+2.5); Met (+1.9); Ala (+1.8); Gly (-0.4); Thr (-0.7); Ser ( 0.8); Trp (-0.9); Tyr (-1.3); Pro (-1.6); His (-3.2); Glu (-3.5); GIn (-3.5); Asp (-3.5); Asn (-3.5); Lys (-3.9); and Arg (-4.5). In alternative embodiments, conserved amino acid substitutions may be 30 made where an amino acid residue is substituted for another in the same class, where the amino acids are divided into non-polar, acidic, basic and neutral classes, -15- WO 2004/026895 PCT/CA2003/001389 as follows: non-polar: Ala, Val, Leu, Ile, Phe, Trp, Pro, Met; acidic: Asp, Glu; basic: Lys, Arg, His; neutral: Gly, Ser, Thr, Cys, Asn, Gin, Tyr. Conservative amino acid changes can include the substitution of an L-amino 5 acid by the corresponding D-amino acid, by a conservative D-amino acid, or by a naturally-occurring, non-genetically encoded form of amino acid, as well as a conservative substitution of an L-amino acid. Naturally-occurring non-genetically encoded amino acids include beta-alanine, 3-amino-propionic acid, 2,3-diamino propionic acid, alpha-aminoisobutyric acid, 4-amino-butyric acid, N-methylglycine 10 (sarcosine), hydroxyproline, ornithine, citrulline, t-butylalanine, t-butylglycine, N methylisoleucine, phenylglycine, cyclohexylalanine, norleucine, norvaline, 2 napthylalanine, pyridylalanine, 3-benzothienyl alanine, 4-chlorophenylalanine, 2 fluorophenylalanine, 3-fluorophenylalanine, 4-fluorophenylalanine, penicillamine, 1,2,3,4-tetrahydro-isoquinoline-3-carboxylix acid, beta-2-thienylalanine, methionine 15 sulfoxide, homoarginine, N-acetyl lysine, 2-amino butyric acid, 2-amino butyric acid, 2,4,-diamino butyric acid, p-aminophenylalanine, N-methylvaline, homocysteine, homoserine, cysteic acid, epsilon-amino hexanoic acid, delta-amino valeric acid, or 2,3-diaminobutyric acid. 20 In alternative embodiments, conservative amino acid changes include changes based on considerations of hydrophilicity or hydrophobicity, size or volume, or charge. Amino acids can be generally characterized as hydrophobic or hydrophilic, depending primarily on the properties of the amino acid side chain. A hydrophobic amino acid exhibits a hydrophobicity of greater than zero, and a 25 hydrophilic amino acid exhibits a hydrophilicity of less than zero, based on the normalized consensus hydrophobicity scale of Eisenberg et al. (J. MoL. Bio. 179:125-142, 1984). Genetically encoded hydrophobic amino acids include Gly, Ala, Phe, Val, Leu, lie, Pro, Met and Trp, and genetically encoded hydrophilic amino acids include Thr, His, Glu, Gln, Asp, Arg, Ser, and Lys. Non-genetically 30 encoded hydrophobic amino acids include t-butylalanine, while non-genetically encoded hydrophilic amino acids include citrulline and homocysteine. -16- WO 2004/026895 PCT/CA2003/001389 Hydrophobic or hydrophilic amino acids can be further subdivided based on the characteristics of their side chains. For example, an aromatic amino acid is a hydrophobic amino acid with a side chain containing at least one aromatic or heteroaromatic ring, which may contain one or more substituents such as -OH, 5 SH, -CN, -F, -CI, -Br, -I, -NO 2 , -NO, -NH 2 , -NHR, -NRR, -C(O)R, -C(O)OH, C(O)OR, -C(O)NH 2 , -C(O)NHR, -C(O)NRR, etc., where R is independently (C1-Ce) alkyl, substituted (C1-C6) alkyl, (C1-C6) alkenyl, substituted (C1-C6) alkenyl, (C1-C6) alkynyl, substituted (C1-C6) alkynyl, (C5-C20) aryl, substituted (C5-C20) aryl, (C6-026) alkaryl, substituted (C6-C26) alkaryl, 5-20 membered heteroaryl, substituted 5-20 10 membered heteroaryl, 6-26 membered alkheteroaryl or substituted 6-26 membered alkheteroaryl. Genetically encoded aromatic amino acids include Phe, Tyr, and Tryp, while non-genetically encoded aromatic amino acids include phenylglycine, 2 napthylalanine, beta-2-thienylalanine, 1,2,3,4-tetrahydro-isoquinoline-3-carboxylic acid, 4-chlorophenylalanine, 2-fluorophenylalanine3-fluorophenylalanine, and 4 15 fluorophenylalanine. An apolar amino acid is a hydrophobic amino acid with a side chain that is uncharged at physiological pH and which has bonds in which a pair of electrons shared in common by two atoms is generally held equally by each of the two atoms 20 (i.e., the side chain is not polar). Genetically encoded apolar amino acids include Gly, Leu, Val, lie, Ala, and Met, while non-genetically encoded apolar amino acids include cyclohexylalanine. Apolar amino acids can be further subdivided to include aliphatic amino acids, which is a hydrophobic amino acid having an aliphatic hydrocarbon side chain. Genetically encoded aliphatic amino acids include Ala, 25 Leu, Val, and lie, while non-genetically encoded aliphatic amino acids include norleucine. A polar amino acid is a hydrophilic amino acid with a side chain that is uncharged at physiological pH, but which has one bond in which the pair of 30 electrons shared in common by two atoms is held more closely by one of the atoms. Genetically encoded polar amino acids include Ser, Thr, Asn, and GIn, -17- WO 2004/026895 PCT/CA2003/001389 while non-genetically encoded polar amino acids include citrulline, N-acetyl lysine, and methionine sulfoxide. An acidic amino acid is a hydrophilic amino acid with a side chain pKa value 5 of less than 7. Acidic amino acids typically have negatively charged side chains at physiological pH due to loss of a hydrogen ion. Genetically encoded acidic amino acids include Asp and Glu. A basic amino acid is a hydrophilic amino acid with a side chain pKa value of greater than 7. Basic amino acids typically have positively charged side chains at physiological pH due to association with hydronium ion. 10 Genetically encoded basic amino acids include Arg, Lys, and His, while non genetically encoded basic amino acids include the non-cyclic amino acids ornithine, 2,3,-diaminopropionic acid, 2,4-diaminobutyric acid, and homoarginine. The above classifications are not absolute and an amino acid may be 15 classified in more than one category. In addition, amino acids can be classified based on known behaviour and or characteristic chemical, physical, or biological properties based on specified assays or as compared with previously identified amino acids. Amino acids can also include bifunctional moieties having amino acid-like side chains. 20 Conservative changes can also include the substitution of a chemically derivatised moiety for a non-derivatised residue, by for example, reaction of a functional side group of an amino acid. Thus, these substitutions can include compounds whose free amino groups have been derivatised to amine 25 hydrochlorides, p-toluene sulfonyl groups, carbobenzoxy groups, t-butyloxycarbonyl groups, chloroacetyl groups or formyl groups. Similarly, free carboxyl groups can be derivatized to form salts, methyl and ethyl esters or other types of esters or hydrazides, and side chains can be derivatized to form O-acyl or O-alkyl derivatives for free hydroxyl groups or N-im-benzylhistidine for the imidazole nitrogen of 30 histidine. Peptide analogues also include amino acids that have been chemically altered, for example, by methylation, by amidation of the C-terminal amino acid by an alkylamine such as ethylamine, ethanolamine, or ethylene diamine, or acylation -18- WO 2004/026895 PCT/CA2003/001389 or methylation of an amino acid side chain (such as acylation of the epsilon amino group of lysine). Peptide analogues can also include replacement of the amide linkage in the peptide with a substituted amide (for example, groups of the formula -C(O)-NR, where R is (CI-C6) alkyl, (C1-C6) alkenyl, (CI-C6) alkynyl, substituted 5 (C1-C6) alkyl, substituted (C1-C6) alkenyl, or substituted (C1-C6) alkynyl) or isostere of an amide linkage (for example, -CH 2 NH-, -CH 2 S, -CH 2
    CH
    2 -, -CH=CH- (cis and trans), -C(O)CH 2 -, -CH(OH)CH 2 -, or -CH 2 SO-). The GD2 ligands, peptides and domains of the invention may be covalently 10 linked, for example, by polymerisation or conjugation, to form homopolymers or heteropolymers. Spacers and linkers, typically composed of small neutral molecules, such as amino acids that are uncharged under physiological conditions, can be used. Linkages can be achieved in a number of ways. For example, cysteine residues can be added at the peptide termini, and multiple peptides can be 15 covalently bonded by controlled oxidation. Alternatively, heterobifunctional agents, such as disulfide/amide forming agents or thioether/amide forming agents can be used. The compound can also be linked to a lipid-containing molecule or peptide that can enhance a T cell response. The compound can also be constrained, for example, by having cyclic portions. 20 Peptides or peptide analogues can be synthesised by standard chemical techniques, for example, by automated synthesis using solution or solid phase synthesis methodology. Automated peptide synthesisers are commercially available and use techniques well known in the art. Peptides and peptide 25 analogues can also be prepared using recombinant DNA technology using standard methods. Compounds of the invention can be provided alone or in combination or conjugation with other compounds (for example, toxins, growth factors, anti 30 apoptotic agents, small molecules, peptides, or peptide analogues), in the presence of a liposome, an adjuvant, or any pharmaceutically acceptable carrier, in a form suitable for administration to humans. Conventional pharmaceutical practice may -19- WO 2004/026895 PCT/CA2003/001389 be employed to provide suitable formulations or compositions to administer the compounds to patients suffering from diseases such as cancer. Any appropriate route of administration may be employed, for example, parenteral, intravenous, subcutaneous, intramuscular, intracranial, intraorbital, ophthalmic, intraventricular, 5 intracapsular, intraspinal, intracisternal, intraperitoneal, intranasal, aerosol, or oral administration. Therapeutic formulations may be in the form of liquid solutions or suspensions; for oral administration, formulations may be in the form of tablets or capsules; and for intranasal formulations, in the form of powders, nasal drops, or aerosols. 10 Formulations for parenteral administration may, for example, contain excipients, sterile water, or saline, polyalkylene glycols such as polyethylene glycol, oils of vegetable origin, or hydrogenated napthalenes. Biocompatible, biodegradable lactide polymer, lactide/glycolide copolymer, or polyoxyethylene 15 polyoxypropylene copolymers may be used to control the release of the compounds. Other potentially useful parenteral delivery systems for modulatory compounds include ethylene-vinyl acetate copolymer particles, osmotic pumps, implantable infusion systems, and liposomes. Formulations for inhalation may contain excipients, for example, lactose, or may be aqueous solutions containing, 20 for example, polyoxyethylene-9-lauryl ether, glycocholate and deoxycholate, or may be oily solutions for administration in the form of nasal drops, or as a gel. If desired, treatment with a compound according to the invention may be combined with more traditional therapies for the disease such as, for example, 25 surgery or chemotherapy. For therapeutic or prophylactic compositions, the compounds may be administered to an individual in an amount sufficient to induce the destruction of cells (such as cancer cells) or to stop or slow the destruction of cells (such as in 30 neuroprotective treatments or treatment of pain). Amounts considered sufficient will vary according to the specific compound used, the mode of administration, the stage and severity of the disease, the age, sex, and health of the individual being - 20 - WO 2004/026895 PCT/CA2003/001389 treated, and concurrent treatments. As a general rule, however, dosages can range from about 1 microgram to about 100 mg per kg body weight of a patient for an initial dosage, with subsequent adjustments depending on the patient's response. 5 In the case of vaccine formulations, an immunogenically effective amount of a compound of the invention can be provided, alone or in combination with other compounds, with an adjuvant, for example, Freund's incomplete adjuvant or aluminum hydroxide. The compound may also be linked with a carrier molecule, 10 such as bovine serum albumin or keyhole limpet hemocyanin to enhance immunogenicity. In general, compounds of the invention should be used without causing substantial toxicity. Toxicity of the compounds of the invention can be determined 15 using standard techniques, for example, by testing in cell cultures or experimental animals and determining the therapeutic index, i.e., the ratio between the LD50 (the dose lethal to 50% of the population) and the LD100 (the dose lethal to 100% of the population). In some circumstances however, such as in severe disease conditions, it may be necessary to administer substantial excesses of the 20 compositions. The following examples are intended to illustrate various embodiments and aspects of the invention, and do not limit the invention in any way. 25 Examples -21 - WO 2004/026895 PCT/CA2003/001389 Methods Cells: EL4 murine lymphoma and R1.1 murine lymphoma were grown in RPMI 1640 medium (Life Technologies) supplemented with 5% fetal bovine serum, 2 mM glutamine, 10 mM Hepes and penicillin/streptomycin at 37,C in 5% CO 2 5 humidified atmosphere. A GD2-negative mutant of EL4 was obtained after subcloning of EL4 cells resistant to culture with anti-GD2 mAb 3F8 and rabbit complement. Flow cytometry: 105 cells in 50 pl FACS buffer (PBS, 0.5% BSA, 0.05% 10 NaN 3 ) were stained for 30 minutes on ice with the following ligands: for GD2, fluorescein isothiocyanate (FITC)-conjugated anti-GD2 mAb 3F8; for GM1, FITC conjugated cholera toxin B subunit (Sigma); for GM2, rabbit anti-GM2 mAb NANA (Matreya) followed by FITC-conjugated anti-rabbit antibody (Sigma); for GD3, mouse anti-GD3 mAb (Pharmingen) followed by FITC conjugated anti-mouse 15 antibody (Sigma). Cells were washed twice with FACS buffer and analyzed on a flow cytometer (Becton-Dickinson) using CellQuest software. Detection of GD2 or GM1 by ELISA: 12.5 ng/well of ganglioside (GD2, Advanced Immunochemicals or GM1) were immobilized by drying onto PVC 96 20 well plates (Falcon), followed by blocking with PBS-0.5% BSA for one hour. Then, anti-GD2 mAb 3F8 or biotin-CTB were added for 10 minutes. The plate was washed three times with PBS-0.5% BSA and incubated 60 minutes with horseradish peroxidase (HRP)-conjugated anti-mouse antibody or HRP conjugated-avidin. After three washes with PBS-0.5% BSA and two with PBS, the 25 colorimetric substrate ABTS was added and the plate read at 414 nm on a Biorad 550 plate reader. Co-immunoprecipitation of GD2 and p56Lck: cells (5 x 106 per sample) were washed in PBS, resuspended in 1 ml lysis buffer (150 mM NaCI, 10 mM 30 sodium phosphate pH 7.2, 2 mM EDTA, 50 mM NaF, 1% CHAPS, 200 pM sodium orthovanadate, 1 mM PMSF, 100 pM leupeptin, 1 mM benzamidine, 300 nM aprotinin, 500 nM soybean trypsin inhibitor) and incubated 30 minutes at 4 0 C. - 22 - WO 2004/026895 PCT/CA2003/001389 Supernatants were collected from lysates after centrifugation (16000 g for 15 minutes at 40C). Immunoprecipitations were carried out overnight at 40C using 50 pl protein-G sepharose (Sigma) with either 5 pg of anti-GD2 mAb 3F8 or anti-p56Lck mAb 3A5 (Santa Cruz Biotechnology) or control IgG; or with 50 p1 avidin-agarose 5 (Sigma) and 5 pg of biotin-CTB which is specific for GM1 gangliosides. Immunoprecipitates were washed 5 times with 1 ml of cold lysis buffer containing decreasing concentration of CHAPS detergent and samples were extracted with reduced Laemmli buffer. Samples were then used for western blot analysis (see below), or lipids from the samples were isolated for ganglioside quantification by 10 ELISA (see above). Biochemical analysis: Western blotting: the immunoprecipitated samples were fractionated in SDS-PAGE and transferred to membranes, p 5 6 Lck immunoblotting was done using rabbit polyclonal anti-p56 Lc k antibody. Tyrosine 15 phosphorylation of p 56 Lck and Zap70: cells (5 x 106 per sample) were washed with PBS, resuspended in 5 ml protein-free hybridoma medium containing 0.2% BSA and allowed to rest for 60 minutes at 370C to lower baseline kinase activity. Then, 10 pg Anti-GD2 mAb 3F8 (13 nM concentration) or non-specific mouse IgG were added for 5 and 20 minutes. Samples were then washed in cold PBS, detergent 20 solubilized in lysis buffer and immunoprecipitated using 5 pg of anti-p56Lck mAb 3A5 (Santa Cruz Biotechnology) or anti-Zap70 mAb LR (Santa Cruz Biotechnology). Samples were then analyzed by western blotting using anti phosphotyrosine mAb 4G10. In vitro p 56 Lck kinase activity: p 5 6 Lck immunoprecipitates were incubated for 20 minutes at 370C with p56Lck-specific 25 substrates GAP p62 or Sam68 in a kinase reaction buffer (10 mM MnCl 2 , 20 mM Tris-HCI pH 7.4, 2.5 pM ATP, 20 pCi [y- 3 2 P]ATP). The reaction was stopped by addition of reduced Laemmli buffer and boiling. Phosphorylation of p 5 6 Lck substrates was visualized and quantified by SDS-PAGE followed by analysis on a Storm 840 phosphorimager with ImageQuant software. 30 Effect of exogenous GD2 on p56 Lck in vitro kinase assay: chromatographically purified p 5 6 Lck tyrosine kinase (Upstate Biotechnology) was - 23 - WO 2004/026895 PCT/CA2003/001389 incubated on ice for 10 minutes in kinase buffer (100 mM Tris-HCI, pH 7.2, 125 mM MgCI 2 , 25 mM MnCl 2 , 2mM EGTA, 150 gM ATP, 0.25 mM sodium orthovanadate, 2 mM DTT) with or without -20 fold molar excess of ganglioside and 10 pCi [y 32 P]ATP. Then 10 gg of the Src kinase substrate peptide p34cdc 2 (6-20) (Upstate 5 Biothechnology) was added to the mix and incubated at room temperature for the times indicated, after which the reaction was stopped by addition of 100 iM lodoacetamide and by precipitation of proteins with final 10% TCA. The supernatant (containing the phosphorylated peptide substrate, not precipitated by TCA) was spotted on a P81 paper (Whatman) and washed three times with 0.75% phosphoric 10 acid and once with acetone. The [ 32 ]-peptide content was quantified using a Storm 840 phosphorimager with ImageQuant software. GD2 ligand design and synthesis: Peptides were modeled based on Tenascin-R sequence and on diversity within Tenascin family homologs. Peptides 15 were synthesized with an Advanced Chemtech automatic synthesizer using solid phase Fmoc chemistry. After cleavage from resin and side-chain deprotection, peptides containing terminal cysteines were subjected to cyclization by oxydation at 40C under 02 in 50 mM ammonium bicarbonate, pH 8.5. Peptides were purified (>95%) by HPLC (Varian) using a C-18 preparative column (Phenomenex), and 20 were verified by Mass Spectometry. Assessment of peptide-GD2 interaction by competitive ELISA: ELISAs were as described above, except that peptides (50 jtg/well, in PBS) were added to the wells for 1 hour before anti-GD2 mAb 3F8 or anti-GM1 cholera toxin-B. 25 Selectivity of inhibition is controlled by lack of effect upon GM1-CTB interactions. Intracellular calcium studies: cells (1 x 106 per sample) were washed with Ringer's solution (155 mM NaCI, 4.5 mM KCI, 2 mM CaCl2, 1 mM MgCl 2 , 10 mM D glucose, 5 mM HEPES) and resuspended in 1 ml Ringer's containing 10 jM p 5 6 Lck 30 inhibitor PP1 (BioMol) or vehicle for 15 minutes at 37oC. Then 5 pM Rhod-2AM (Molecular Probes) was then added to the cells and incubated for 30 minutes at room temprature with mild agitation. Cells were washed once in Ringer's, -24- WO 2004/026895 PCT/CA2003/001389 resuspended in 1 ml Ringer's and incubated for 30 minutes, after which cells were stimulated with the indicated ganglioside ligands (or calcium ionophore A23187 as control) and analyzed over a 30 minute period for intracellular Ca 2 + using a flow cytometer (Becton-Dickinson). 5 Soft agar colony formation: single cell suspensions of EL4 GD 2 ne g ative and EL4 GD2positive were plated (1500 cells/plate) with or without mAb 3F8 ( 50 pg/plate) in top layer medium (RPMI 1640 containing 0.35% agar, 15% fetal bovine serum, 2 mM glutamine, 10 mM Hepes and penicillin/streptomycin) on top of a preformed 10 bottom layer (same as above except 0.5% agar) in 100 mm polystyrene dishes (Falcon) and grown until colonies are visible by eye. Colonies were counted in a predetermined area of the plates. In vivo tumor studies: single cell suspensions of EL4 GD 2 ne g at i ve and EL4 15 GD2positive in Hanks' balanced salt solution (Gibco) were injected intraperitonially in nude Balb/c mice (Charles River) (1 x 106 cells per animal). Animals were sacrificed and autopsied after 7 to 10 days, depending on tumor growth. In vitro CD45 phosphatase assay: human recombinant CD45 enzyme 20 (BioMol, 75 units/well) was preincubated in the absence (negative control) or presence of sodium orthovanadate (positive control) or various gangliosides (16.7 pM) for 20 minutes at room temperature in half-volume 96-well microtiter plates. A CD45-specific substrate (pp 6 0src C-terminal phosphoregulatory peptide, 200 pM) was then added for 20 minutes at 30'C, afterwhich the reaction was stopped and 25 quantified with BioMol Green reagent. Plates were read at 620 nm with a Biorad 550 platew reader. Ex vivo CD45 assay: single cell suspensions of EL4 GD 2 ne ga ti ve and EL4
    GD
    2 positive (1 x 107 cells/ml per treatment) were incubated in the presence of 5 30 pg/ml of mlg (negative control), anti-GD2 antibody 3F8 or anti-CD3 antibody 145 2C1 I (Pharmingen) cross-linked with anti-hamster G94-56 (Pharmingen) for 20 minutes at 37°C in RPMI 1640 medium supplemented with 5% fetal bovine serum, -25- WO 2004/026895 PCT/CA2003/001389 2 mM glutamine, 10 mM Hepes and penicillin/streptomycin. Cells were then washed and detergent lysed. p 5 6 Lck was then immunoprecipitated with anti-p56Lck antibody coated beads and probed for phosphotyrosine 505 by western blotting with anti-PY505 (Cell Signaling Technology). 5 Results To study a specific physical association between p 5 6 Lck and gangliosides, a panel of cells was generated varying in expression of cell surface GD2, GM1, GM2 and GD3, as assessed by flow cytometry. The indicated cells (Table 2) express 10 similar levels of p 5 6 Lck (data not shown). Table 2 Surface ganglioside expression of cells studied as assessed by FACScan Cells Source Ganglioside profile p56 L c k GM1 GD2 GD3 GM2 expression EL4 GD2posItve lymphoma mouse C57/bl6n Low high negative negative High EL4 GD2negative lymphoma mouse C57/bl6n Low negative medium negative High R1.1 lymphoma mouse C58/J High low negative negative High 15 Specific and stable association of p56Lck--GD2. We performed anti-GD2 immunoprecipitations with mAb 3F8 followed by western blotting with anti-p56Lck antibodies (Figure 2A). In EL4 GD2positive cells p 5 6 Lck was co-precipitated with mAb 3F8 but not with control mouse IgG. In control RI.1 cells, which are GD2negative , no antibody co-precipitated p 5 6 Lck , although p 5 6 Lck 20 is expressed at high levels. The presence and kinase activity of p 5 6 Lck in anti-GD2 immunoprecipitates were verified and quantified (Figures 2B and 2C). The indicated immunoprecipitations were done on EL4 GD 2 po siti ve or EL4 GD 2 negative cells, followed 25 by in vitro kinase assays using the specific p 5 6 Lck substrate Sam68. In EL4 GD2positive approximately 15% of the p 5 6 Lck activity was co-precipitated by anti-GD2 mAb 3F8 (Figure 2B, lane 1), compared with anti-Lck mAb 3A5 (Figure 2B, lane 2). -26- WO 2004/026895 PCT/CA2003/001389 In fact, anti-GD2 co-precipitation of the p 5 6 Lck activity was comparable to co precipitation by antibodies against Zap70 (Figure 2B, lane 3). This was in keeping with the fact that Zap70 and p 56 Lck are reportedly associated physically and functionally in vivo. 5 The specificity of these assays is validated in studies using EL4 GD2nega ti ve cells, where anti-GD2 mAb 3F8 did not co-precipitate a p 56 Lck activity (Figure 2B, lane 4), while anti-Lck and anti-Zap70 antibodies did (Figure 2B, lanes 5 and 6). Because p 5 6 Lck co-immunoprecipitations with anti-Zap70 antibodies were 10 comparable in EL4 GD2positive and EL4 GD2ne g ative cell lines (Figure 2B, lanes 3 and 6), the data suggest that association of Zap70 and p 5 6 Lck can be GD2 independent. Similar studies were done using EL4 GD 2
    PO
    s iti ve or R1.1 (GD 2 ne g at i ve) cells and assaying a p 5 6 Lck activity by in vitro kinase activity upon the selective substrate 15 Gap62 (Figure 2C). Again, anti-GD2 co-purified a p 5 6 Lck activity that corresponds to -15% of the activity purified with anti-Lck mAb 3A5. In contrast, affinity isolation of GM1 with cholera toxin b subunit-coupled beads did not co-purify a p 5 6 Lck activity (Figure 2C, lane 4), although R1.1 cells have high levels of GM1 and p 56 Lck (Figure 2C, lane 3). 20 The converse experiment was performed where p 5 6 Lck was first immunoprecipitated with mAb 3A5 and then the presence of GD2 or GM1 were gauged by ELISA (Figure 2D). Anti-p56Lck immunoprecipitates from EL4 GD2OSit i ve cells contained -55% of the GD2 that could be isolated with anti-GD2 mAb 3F8. In 25 specificity controls, anti-p56Lck immunoprecipitates from R1.1 cells did not co precipitate the ganglioside GM1. Functional relevance of p 5 6 LCk-GD2 association in the absence of GD2 ligands. 30 Subsequent experiments (Figure 3) addressed in vitro whether there is a functional relevance to the stable and selective GD2-p56Lck association. Addition of exogenous GD2 to purified p 5 6 Lck increased the kinetics of in vitro enzymatic -27 - WO 2004/026895 PCT/CA2003/001389 activity from a tl/ 2 of -25 minutes to a t 1 1
    /
    2 of -17 minutes, with a dramatic 70% increase in kinase activity at 20 minutes. However, the Vmax did not change, as the enzyme activity reaches a similar plateau with or without GD2 present. In control assays, p 5 6 Lck kinase activity was not altered upon addition of exogenous GM1 5 (Figure 3), or addition of phosphatidylcholine or other lipids (data not shown). Development of artificial GD2 ligands. Small peptides (6-13 amino acids in length) were designed to span the primary sequence of the GD2 ligand Tenascin-R. Cyclization and other 10 conformational constrains were introduced in these peptides to conformationally constrain them.and to forcethem to adopt the j3-turn structures desired. These peptides (~100 were synthesized) were tested for inhibition of GD2 mAb 3F8 interactions in ELISA plates (Table 3). No competitor peptide added or 15 control irrelevant peptide added were standardized as 100% binding. For simplicity, only some of the -90 inactive peptides are shown. In various embodiments, peptide analogs that actively inhibit GD2-mAb 3F8 interactions span the sequence NH 2 -lle-Thr(Ala)-Asn-Tyr-Asn-COOH. In some 20 embodiments, peptides of the invention may be in type IV (3:5 or 3:3) canonical P3 turn configurations with a distance between the Cal to Ca4 atom varying between 4 and 7 A, depending on whether Gly residues (underlined) are incorporated in the sequence. Additionally, a linear peptide of 11 amino acids (pep 51), which is unstructured in solution (data not shown) can also act as a competitive inhibitor. 25 - 28 - WO 2004/026895 PCT/CA2003/001389 Table 3. Selected peptide mimics.. Peptides Sequence Conformation % Inhibition of mAb 3F8-GD2 interactions Pep 51 GG I T N Y N S A L M linear 44.96 ± 1.57 Pep 52 C G GITNYNSAC cyclic 30.17 ± 2.69 Pep53 CITNYNSC cyclic 24.74 ± 3.29 Pep 54 C GG I T N Y N C cyclic 40.34 ± 3.17 Pep 55 CTNY G V HC cyclic 12.23 ± 4.41 Pep 56 CTNY G VC cyclic 14.13 ± 5.43 Pep 57 GGIANYNTS linear 4.33 ± 5.23 Pep 58 C G GIANY NC cyclic 47.60 ± 4.26 Pep 59 C G GIANYNTSC cyclic 48.83 ± 5.01 Pep 60 CIANYNTC cyclic 30.94 ± 3.53 Inhibition of mAb 3F8 bindng to immobilized GD2 by peptides, measured by competitive ELISA. No peptide or control peptide treatments are standardized as 5 0% inhibition. No mAb 3F8 treatment is standardized as 100% inhibition. Average of 5 indepedant experiments, n=4 in each experiment. Mean ± SEM. Functional relevance of GD2 ligands in p 5 6 Lck activation. This example illustrates (i) whether engagement of cell surface GD2 with 10 ligands causes the pTyr of p 5 6 Lck (Figure 4A); (ii) whether engagement of cell surface GD2 with ligands leads to the pTyr of the downstream effector Zap70 (Figure 4B); (iii) whether engagement of cell surface GD2 induces intracellular calcium fluxes (Figure 4C). 15 First, engagement of cell surface GD2 in living EL4 GD2po s i tive cells with the artificial GD2 ligand mAb 3F8 caused the pTyr of p 5 6 Lck within 5 min in vivo (Figure 4A, lanes 3 and 4). Control mouse IgG did not cause this effect (Figure 4A, lanes 1 and 2). - 29 - WO 2004/026895 PCT/CA2003/001389 Second, since the pTyr of p 5 6 Lck can lead either to activation or to inactivation of this kinase depending on which Tyr residue gets phosphorylated, the pTyr profile of Zap70 was studied in vivo because it is an adapter molecule downstream of and phosphorylated by activated p 5 6 Lck . Zap70 is transiently pTyr 5 upon engagement of cell-surface GD2 with mAb 3F8 as a ligand (Figure 4B, lanes 3 and 4). This Zap70 pTyr presumably occurs via p 5 6 Lck . Control mouse IgG did not cause Zap70 pTyr in EL4 GD2poSitve cells (Figure 4B, lanes 1 and 2). In other specificity controls, treatment of EL4 GD2negative cells with GD2 ligands did not cause pTyr of p 5 6 Lck or Zap70 (data not shown) indicating that cell surface GD2 10 expression may be required for ligand engagement of GD2 and a subsequent effect downstream, in some embodiments. Third, intracellular Ca ++ concentrations were measured in live EL4 GD 2 po s iti ve cells after engagement of cell-surface GD2 with ligands (Figure 4C). Flow 15 cytometric analysis using the Ca ++ sensitive Rhod-2 fluorophore showed a rapid (~5 minutes) 1.6 fold increase of intracellular Ca ++ after mAb 3F8 binding, but not after binding by control mouse antibody or CTB (data not shown). The selective p 5 6 Lck kinase inhibitor PP1 (10 pM) markedly decreased mAb 3F8-induction of Ca" + fluxes. This control suggests that activated p 5 6 Lck may be responsible for aspects 20 of the GD2 signal transduction pathway leading to intracellular Ca ++ fluxes in some embodiments. Functional relevance of the presence of GD2 on CD45 activity. Since the CD45 phosphatase is responsible for the activation of p 5 6 Lck , the 25 effect of GD2 on CD45 activity was investigated. First, the effect of GD2 was tested in vitro using human recombinant CD45 enzyme and pp60 s rc phosphoregulatory peptide as a substrate. As seen in figure 5, GD2 can drastically inhibit CD45 phosphatase activity (85% inhibition), while other gangliosides (GM1, GM2, GD3 and GM3) had little influence on the enzymatic activity of CD45. Other than GD2, 30 only GD1a showed significant inhibition (50%). - 30 - WO 2004/026895 PCT/CA2003/001389 The inhibitory effect of GD2 on CD45 is also seen in live cells, as shown in figure 6. Here, EL4 GD 2 posit i ve and EL4 GD 2 negative cells were stimulated using the well-documented T cell receptor cross-linking methodology, which typically results in activation of p 5 6 Lck following its dephosphorylation at tyrosine 505 by CD45. 5 Interestingly, EL4GD2posi ti ve cells are resistant to activation (Figure 6, lane 3), while the EL4 GD2ne g ative cells (Figure 7, lane 6) are readily activated upon T cell receptor cross-linking. This discrepancy can be attributed to the presence of GD2 in EL4 GD2po s i tive cells which can block CD45-mediated activation of p 5 6 Lck . Of interest is the fact that GD2 ligands, such as mAb 3F8, seem to be able to alleviate GD2 10 inhibition of CD45 and allow for T cell receptor activation as seen by dephosphorylation of tyrosine 505 on p 5 6 Lck (Figure 6, lane 2). Functional relevance of GD2 and GD2 ligands in tumorigenic growth. To illustrate the effect of cell surface expression of GD2 on cancer cell 15 growth and survival, EL4 GD 2 po s i ti ve and EL4 GD 2 ne g at i ve cells were grown in clonoenic (soft agar) assays (Figure 7), or they were injected in vivo intraperitoneally (Figure 8). Colony formation assays in soft agar showed marked differences in growth 20 dynamics for EL4 GD2positive and EL4 GD 2 negative cells. At day 10 of growth, the total number of EL4 GD 2 po si tve colonies per plate were lower by 50% compared to EL4
    GD
    2 ne g ative colonies (Figure 7a). Moreover the EL4 GD 2 ne g ative colonies were much larger and contained more cells (Figure 7b). This is striking because both cell lines have identical doubling times in liquid culture. 25 Addition of mAb 3F8 in the agar layer (where it putatively diffuses, engages and activates GD2-mediated signals) totally abolished EL4 GD2PO s i tive colony formation while having no consequences on the number and size of EL4 GD 2 negative colonies. This illustrates that the GD2 ligands of the invention may be used to treat 30 GD2 positive cells to modulate growth, for example to treat cancer cells expressing GD2 to inhibit the growth of such cells or to kill the cells. In some embodiments, EL4 GD2po si tive cells die by apoptosis when GD2 is bound (data not shown), -31 - WO 2004/026895 PCT/CA2003/001389 indicating that the presence of GD2 may in some embodiments allow the GD2 ligands of the invention to be used to induce apoptosis. An evident difference is also observed when cells are grown in vivo. When 5 injected intraperitoneally EL4 GD 2 ne g at i ve cells mainly form aggressive and metastatic ascitic tumors and the peritoneal cavity contains mucin-like peptidoglycans. In contrast, EL4 GD 2 p s itive cells form localized, highly vascularized solid tumors attached to the peritoneal membrane (Figure 8). 10 Conclusion Although various embodiments of the invention are disclosed herein, many adaptations and modifications may be made within the scope of the invention in accordance with the common general knowledge of those skilled in this art. Such modifications include the substitution of known equivalents for any aspect of the 15 invention in order to achieve the same result in substantially the same way. Numeric ranges are inclusive of the numbers defining the range. In the specification, the word "comprising" is used as an open-ended term, substantially equivalent to the phrase "including, but not limited to", and the word "comprises" has a corresponding meaning. Citation of references herein shall not be construed 20 as an admission that such references are prior art to the present invention. All publications, including but not limited to patents and patent applications, cited in this specification are incorporated herein by reference as if each individual publication were specifically and individually indicated to be incorporated by reference herein and as though fully set forth herein. The invention includes all 25 embodiments and variations substantially as hereinbefore described and with reference to the examples and drawings. References The following documents are hereby incorporated by reference: 30 -32 - WO 2004/026895 PCT/CA2003/001389 Cheung, N. K., Saarinen, U. M., Neely, J. E., Landmeier, B., Donovan, D., and Coccia, P. F. (1985). Monoclonal antibodies to a glycolipid antigen on human neuroblastoma cells, Cancer Research 45, 2642-9. 5 Eisenberg, D., Schwarz, E., Komaromy, M., Wall, R. (1984). Analysis of membrane and surface protein sequences with the hydrophobic moment plot, Journal of Molecular Biology 179,125-42. Gagnon, M., and Saragovi, H. U. (2002). Gangliosides: therapeutic agents or 10 therapeutic targets , Expert Opinion on Therapeutic Patents 12, 1215-1224. Probstmeier, R., Michels, M., Franz, T., Chan, B. M., and Pesheva, P. (1999). Tenascin-R interferes with integrin-dependent oligodendrocyte precursor cell adhesion by a ganglioside-mediated signalling mechanism, European Journal of 15 Neuroscience 11, 2474-88. Saragovi, H. U., Greene, M. I., Chrusciel, R. A., and Kahn, M. (1992). Loops and secondary structure mimetics: development and applications in basic science and rational drug design, BiolTechnology 10, 773-8. 20 Sorkin, L. S. (2000). Antibody activation and immune reactions: potential linkage to pain and neuropathy, Pain Medicine 1, 296-302. 25 - 33 -
    权利要求:
    Claims (26)
    [1] 1. A substantially pure GD2 ligand of Formula I: 5 Zl-X 1 -X
    [2] 2 -X 3 -X 4 -X 5 -X 6 -X 7 -X-X 9 -Xo 0 -X 1 -X 12 -X 13 -Z 2 (I) wherein X, is absent or Y or an analogue thereof; 10 X 2 is absent or C or an analogue thereof; X 3 is G or Y or an analogue thereof; X 4 is G or C or Y or an analogue thereof; X 5 is I or C or an analogue thereof; X 6 is T or A or an analogue thereof; 15 X 7 is N or an analogue thereof; X 8 is Y or an analogue thereof; X 9 is N or G or an analogue thereof; X1o is S or C or V or T or an analogue thereof; X 11 is A or C or Y or H or S or an analogue thereof; 20 X 12 is absent or L or C or Y or an analogue thereof; X 13 is absent or M or Y or an analogue thereof; Zl is an N-terminal group of the formula H2N-, RHN- or, RRN-; Z 2 is a C-terminal group of the formula -C(O)OH, -C(O)R, -C(O)OR, C(O)NHR, -C(O)NRR; 25 R at each occurrence is independently selected from (Cl-C6) alkyl, (C-C6) alkenyl, (C 1 -C 6 ) alkynyl, substituted (C 1 -C 6 ) alkyl, substituted (Cl-C6) alkenyl, or substituted (Cl-C 6 ) alkynyl; and wherein "-" is a covalent linkage. 30 2. A substantially pure synthetic GD2 ligand or recombinant GD2 ligand having a domain of Formula 11: -X 1 -X 2 -X 3 - X 4 - X5 - X6 - X 7 - X8 - X 9 -XX 10 - X 1 - X 12 - X 13 - (11) -34- WO 2004/026895 PCT/CA2003/001389 wherein X 1 is absent or Y or an analogue thereof; X 2 is absent or C or an analogue thereof; 5 X 3 is G or Y or an analogue thereof; X 4 is G or C or Y or an analogue thereof; X 5 is I or C or an analogue thereof; X 6 is T or A or an analogue thereof; X 7 is N or an analogue thereof; 10 X 8 is Y or an analogue thereof; X 9 is N or G or an analogue thereof; X 10 is S or C or V or T or an analogue thereof; X 1 , is A or C or Y or H or S or an analogue thereof; X 12 is absent or L or C or Y or an analogue thereof; 15 X 13 is absent or M or Y or an analogue thereof; and wherein "-" is a covalent linkage.
    [3] 3. The GD2 ligand of claim 1 or 2, wherein the ligand further comprises a cyclic linkage between any two of X 1 through X 13 . 20
    [4] 4. The GD2 ligand of claim 1, wherein the ligand is selected from the group consisting of: GGITNYNSALM; YCGGITNYNSACY; YCITNYNSCY; YCGGITNYNCY; YCTNYGVHCY; YCTNYGVCY; GGIANYNTS; YCGGIANYNCY; YCGGIANYNTSCY; and, 25 YCIANYNTCY.
    [5] 5. The GD2 ligand of claim 2, wherein the domain is selected from the group consisting of: GGITNYNSALM; YCGGITNYNSACY; YCITNYNSCY; YCGGITNYNCY; YCTNYGVHCY; YCTNYGVCY; 30 GGIANYNTS; YCGGIANYNCY; YCGGIANYNTSCY; and, YCIANYNTCY. - 35 - WO 2004/026895 PCT/CA2003/001389
    [6] 6. A method of treating a subject having a disease wherein disease cells express GD2, the method comprising administering to the subject an effective amount of the GD2 ligand of any one of claims 1 through 5. 5
    [7] 7. A method of diagnosis of a disease wherein disease cells express GD2, comprising determining whether a cell from a subject binds to the GD2 ligand of any one of claims 1 through 5.
    [8] 8. The method of claim 6 or 7 wherein the method is carried out in vitro. 10
    [9] 9. The method of claim 6 or 7 wherein the method is carried out in vivo.
    [10] 10. The method of claim 6, further comprising administering to the patient an effective amount of granulocyte-macrophage colony-stimulating 15 factor.
    [11] 11. A pharmaceutical composition comprising the GD2 ligand of any one of claims 1 through 5, together with an effective amount of granulocyte-macrophage colony-stimulating factor. 20
    [12] 12. A commercial package comprising the GD2 ligand of any one of claims 1 through 5, together with instructions for using the GD2 ligand to modulate GD2 activity or detect cells expressing GD2. 25
    [13] 13. The GD2 ligand of claim 2, wherein the GD2 ligand is a recombinant T-cell receptor.
    [14] 14. The GD2 ligand of claim 13, wherein the recombinant T-cell receptor is expressed in a cytotoxic T cell line. 30
    [15] 15. An isolated GD2 ligand substantially as hereinbefore described and with reference to the examples. - 36 - WO 2004/026895 PCT/CA2003/001389
    [16] 16. A method of ablating a cell line, comprising transforming the cell line to provide transformed cells that express GD2, and treating the transformed cells with an effective amount of the GD2 ligand of claim 5 1 or2.
    [17] 17. The use of a GD2 ligand of any one of claims 1 through 5 to formulate a medicament to treat a disease wherein diseases cells express GD2. 10
    [18] 18. The use of a GD2 ligand according to claim 17, wherein the disease is a cancer.
    [19] 19. The use of a GD2 ligand according to claim 18, wherein the cancer is a neuroblastoma. 15
    [20] 20. A method of screening to identify or validate a putative GD2 ligand, comprising: a) administering a putative GD2 ligand to a system having a GD2 moiety and a p 5 6 Lck moiety available for association; and, 20 f) measuring an association or functional relationship between the GD2 and the p 5 6 Lck moieties in the system.
    [21] 21. The method of claim 20, wherein the putative GD2 ligand comprises a polypeptide or a non-peptidic analog such as a peptidomimetic that 25 displays the same pharmacophore or has similar side chain functional groups.
    [22] 22. The method of claim 20, wherein the putative GD2 ligand is derived from tenascin-R. 30
    [23] 23. The method of claim 20, wherein the system is a cell expressing GD2 and p 5 6 Lck - 37 - WO 2004/026895 PCT/CA2003/001389
    [24] 24. The method of claim 20, wherein the GD2 moiety is native GD2.
    [25] 25. The method of claim 20, wherein the p56Lok moiety is native p56Lck 5
    [26] 26. The method of claim 20, wherein the association between the GD2 and the p 5 6 Lck moieties is measured by determining a kinase activity of the p 5 6 Lck moiety. 10 -38-
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    同族专利:
    公开号 | 公开日
    WO2004026895A2|2004-04-01|
    JP2006516241A|2006-06-29|
    US20060159652A1|2006-07-20|
    US7423114B2|2008-09-09|
    WO2004026895A3|2004-08-12|
    CA2538719A1|2004-04-01|
    EP1543022A2|2005-06-22|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US5935821A|1995-01-17|1999-08-10|Board Of Trustees Of The University Of Kentucky|Polynucleotides related to monoclonal antibody 1A7 and use for the treatment of melanoma and small cell carcinoma|US7432251B2|2005-03-14|2008-10-07|Institut Pasteur|Methods of treating neurological diseases by regulating migration of neuroblasts in the adult nervous system with tenascin-R|
    CA2793276A1|2009-03-27|2010-09-30|Zacharon Pharmaceuticals, Inc.|Ganglioside biosynthesis modulators|
    AU2011268110B2|2010-06-19|2016-05-19|Memorial Sloan-Kettering Cancer Center|Anti-GD2 antibodies|
    KR102207859B1|2013-03-15|2021-01-27|메모리얼 슬로안 케터링 캔서 센터|High affinity anti-gd2 antibodies|
    法律状态:
    2007-09-27| MK1| Application lapsed section 142(2)(a) - no request for examination in relevant period|
    优先权:
    申请号 | 申请日 | 专利标题
    US41249202P| true| 2002-09-20|2002-09-20||
    US60/412,492||2002-09-20||
    PCT/CA2003/001389|WO2004026895A2|2002-09-20|2003-09-19|Gd2 ligands|
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